主要研究领域有：（1）真核细胞中RNA的表达与加工的调节；（2）真核生物中转录调节机制；（3）非编码RNA的表达与调节机制；（4）模式生物的遗传与发育；(5) 基因编辑和染色体操纵的新技术和新方法。
1996年在中国科学技术大学生物系获得学士学位。1996-1999年师从中国科大施蕴渝院士进行多肽的溶液构象研究 并获得硕士学位。1999-2004年，在美国威斯康星大学癌症研究所师从Janet Mertz教授，研究真核生物中病毒起源的无内含子RNA的表达和调控机制，并获得博士学位。2005-2010年，作为博士后在威斯康星大学遗传系Scott Kennedy教授实验室，从事模式生物中小干扰RNA的功能和调控机理的研究。2010年,加入生命科学院，进一步研究非编码RNA的功能和调控机制。以第一和通讯作者在包括《Nature》和《Science》在内的国际著名期刊上发表论文。
主要研究成果：
确认了高等动物中存在着细胞核里的基因干扰现象；发现了siRNA从细胞质转运到细胞核的途径；发现和解析了nrde通路；发现细胞核RNA干扰可以抑制RNA聚合酶II的转录延伸，造成转录的提前终止；发现高等动物中siRNA造成组蛋白的H3K9me3和H3K27me3甲基化(Science, 2008；Nature, 2010；PLoS Genetics，2011；Genetics, 2014；Curr. Biol., 2015)；发现细胞核和细胞质RNAi干扰通路都参与了生物体获得性性状的多代遗传(Nature Genetics，2012；Cell Reports, 2018）；发现了核仁RNA 干扰，发现了一类受环境刺激调控的针对rRNA的小干扰RNA(risiRNA)和抑制risiRNA产生的Susi通路，发现了一种新的rRNA稳态调节机制(Nat Struct Mol Biol, 2017；PNAS, 2018；Nucleic Acids Research, 2021a)；发现和解析了介导piRNA转录起始的USTC复合物和帮助piRNA转录后加工的PICS复合物(Genes & Development, 2019；Cell Reports, 2019；PNAS, 2021；Nature Communications, 2021；JGG, 2022)；发展了基因编辑的新方法 (Sci. Rep., 2014；Genetics,2015; G3, 2018）。
代表性学术论文：
1. Minjie Hong(#), Xiaotian Zhou(#), Chenming Zeng, Demin Xu, Ting Xu, Shimiao Liao, Ke Wang, Chengming Zhu, Ge Shan, Xinya Huang(*), Xiangyang Chen(*), Xuezhu Feng(*) & Shouhong Guang(*) (2024b) Nucleolar stress induces nucleolar stress body formation via the NOSR-1/NUMR-1 axis in Caenorhabditis elegans. Nature Communications, 2024 volume 15, Aug 23 https://doi.org/10.1038/s41467-024-51693-z
2. Xiangyang Chen(#), Ke Wang(#), Farees Ud Din Mufti(#), Demin Xu, Chengming Zhu, Xinya Huang, Chenming Zeng, Qile Jin, Xiaona Huang, Yong-hong Yan, Meng-qiu Dong, Xuezhu Feng(*), Yunyu Shi(*), Scott Kennedy(*) & Shouhong Guang(*) (2024a) Germ granule compartments coordinate specialized small RNA production. Nature Communications, 15, 5799 (2024). https://doi.org/10.1038/s41467-024-50027-3
3. Ling Liu, Yifan Wu, Ke Liu, Mengdan Zhu, Shouhong Guang, Fengsong Wang, Xing Liu, Xuebiao Yao, Jiajia He(*), Chuanhai Fu(*) (2024) The absence of the ribosomal protein Rpl2702 elicits the MAPK-mTOR signaling to modulate mitochondrial morphology and functions. Redox Biol. 2024 Apr 29:73:103174. doi: 10.1016/j.redox.2024.103174.
4. Wei I Jiang, Henry De Belly, Bingying Wang, Andrew Wong, Minseo Kim, Fiona Oh, Jason DeGeorge, Xinya Huang, Shouhong Guang, Orion D Weiner, Dengke K Ma (2024) Early-life stress triggers long-lasting organismal resilience and longevity via tetraspanin Science Advance 2024 Jan 26;10(4):eadj3880. doi: 10.1126/sciadv.adj3880. Epub 2024 Jan 24.
5. Ruofan Ding, Xudong Zou, Yangmei Qin, Lihai Gong, Hui Chen, Xuelian Ma, Shouhong Guang, Chen Yu, Gao Wang, Lei Li (2023) xQTLbiolinks: a comprehensive and scalable tool for integrative analysis of molecular QTLs. Brief Bioinform. 2023 Nov 22;25(1):bbad440. doi: 10.1093/bib/bbad440.
6. Pandey T, Kalluraya CA, Wang B, Xu T, Huang X, Shouhong Guang, Daugherty MD, Ma DK (*) (2023) Acquired stress resilience through bacteria-to-nematode interdomain horizontal gene transfer. EMBO J. (2023b) Nov 13:e114835. doi: 10.15252/embj.2023114835.
7. Zhiwen Zhu(#), Dongdong Li, Zeran Jia, Wenhao Zhang, Yuling Chen, Ruixue Zhao, Yan-Ping Zhang, Wen-Hong Zhang, Haiteng Deng, Yinqing Li, Wei Li, Shouhong Guang, Guangshuo Ou(*) (2023) Global histone H2B degradation regulates insulin/IGF signaling-mediated nutrient stress. The EMBO Journal (2023a) Aug 29:e113328. doi: 10.15252/embj.2022113328.
8. Demin Xu(#), Xiangyang Chen(#), Yan Kuang, Minjie Hong, Ting Xu, Ke Wang, Xinya Huang, Chuanhai Fu, Ke Ruan, Chengming Zhu(*), Xuezhu Feng(*) and Shouhong Guang(*) (2023) rRNA intermediates coordinate the formation of nucleolar vacuoles in C. elegans. Cell Reports, 2023 Aug 1;42(8):112915. doi: 10.1016/j.celrep.2023.112915.
9. Xinhao Hou(#), Mingjing Xu(#), Chengming Zhu(#), Jianing Gao, Meili Li, Xiangyang Chen, Cheng Sun, Björn Nashan, Jianye Zang, Ying Zhou(*), Shouhong Guang(*), and Xuezhu Feng(*) (2022) Systematic characterization of chromodomain proteins reveals an H3K9me1/2 reader regulating aging in C. elegans. Nature Communications, 2023 Mar 6;14(1):1254. doi: 10.1038/s41467-023-36898-y.
10. Ting Xu，Shimiao Liao , Meng Huang, Chengming Zhu, Xiaona Huang, Qile Jin, Demin Xu, Chuanhai Fu, Xiangyang Chen(*), Xuezhu Feng(*) and Shouhong Guang(*) (2023) A ZTF-7/RPS-2 complex mediates the cold-warm response in C. elegans. PLoS Genetics, 2023 Feb 10;19(2):e1010628. doi: 10.1371/journal.pgen.1010628
11. Meng Huang(#), Minjie Hong(#), Chengming Zhu, Di Chen(*), Xiangyang Chen(*), Shouhong Guang(*), and Xuezhu Feng(*) (2022) H3K9me1/2 methylation limits the lifespan of daf-2 mutants in C. elegans. Elife. 2022 Sep 20;11:e74812. doi: 10.7554/eLife.74812.
12. Xuelian Ma(#), Shumin Cheng(#), Ruofan Ding, Zhaozhao Zhao, XuDong Zou, Shouhong Guang, Qixuan Wang, Huan Jing, Chen Yu, Ting Ni, Lei Li(*) (2022) ipaQTL-atlas: an atlas of intronic polyadenylation quantitative trait loci across human tissues. Nucleic Acids Research, 2022 Aug 31, https://doi.org/10.1093/nar/gkac736
13. Xinhao Hou(#), Chengming Zhu, Mingjing Xu, Xiangyang Chen, Cheng Sun, Björn Nashana(*), Shouhong Guang(*), and Xuezhu Feng(*) (2022) The SNAPc complex mediates starvation-induced trans-splicing in C. elegans. Journal of Genetics and Genomics, 2022 Mar 10:S1673-8527(22)00076-5. doi: 10.1016/j.jgg.2022.02.024.
14. Xiaoyang Wang(#), Chenming Zeng(#), Shanhui Liao(#), Zhongliang Zhu, Jiahai Zhang, Xiaoming Tu, Xuebiao Yao, Xuezhu Feng(*), Shouhong Guang(*), and Chao Xu(*) (2021) Molecular basis for PICS-mediated piRNA biogenesis and cell division. Nature Communications, 2021 Sep 22;12(1):5595. doi: 10.1038/s41467-021-25896-7
15. Shimiao Liao(#), Xiangyang Chen(#), Ting Xu(#), Qile Jin, Zongxiu Xu, Demin Xu, Xufei Zhou, Chengming Zhu(*), Shouhong Guang(*) and Xuezhu Feng(*) (2021a) Antisense ribosomal siRNAs inhibit RNA polymerase I-directed transcription in C. elegans Nucleic Acids Research 2021 Sep 20;49(16):9194-9210. doi: 10.1093/nar/gkab662.
16. Zheng Xu(#), Jie Zhao, Minjie Hong, Chenming Zeng, Shouhong Guang(*), Yunyu Shi(*) (2021b) Structural recognition of the mRNA 3’ UTR by PUF-8 restricts the lifespan of C. elegans. Nucleic Acids Research, 2021 Sep 27;49(17):10082-10097. doi: 10.1093/nar/gkab754
17. Xinya Huang(#), Peng Cheng(#), Chenchun Weng, Zongxiu Xu, Chenming Zeng, Xiangyang Chen(*), Chengming Zhu(*), Shouhong Guang(*), and Xuezhu Feng(*) (2021) A chromodomain protein mediates heterochromatin-directed piRNA expression. PNAS, 2021 Jul 6;118(27):e2103723118. doi: 10.1073/pnas.2103723118
18. Chenming Zeng (#), Chenchun Weng (#), Xiaoyang Wang (#), Yong-Hong Yan (#), Wen-Jun Li, Demin Xu, Minjie Hong, Shanhui Liao, Meng-Qiu Dong, Xuezhu Feng (*), Chao Xu (*), and Shouhong Guang (*) (2019) Functional proteomics identifies a PICS complex required for piRNA maturation and chromosome segregation. Cell Reports Volume 27, ISSUE 12, P3561-3572.e3, June 18, 2019, DOI: https://doi.org/10.1016/j.celrep.2019.05.076
19. Chenchun Weng (#), Joanna Kosalka (#), Ahmet C. Berkyurek (#), Przemyslaw Stempor, Xuezhu Feng, Hui Mao, Chenming Zeng, Wen-Jun Li, Yong-Hong Yan, Meng-Qiu Dong, Natalia Rosalía Morero, Cecilia Zuliani, Orsolya Barabas, Julie Ahringer, Shouhong Guang (*), and Eric A. Miska (*) (2018) The USTC complex co-opts an ancient machinery to drive piRNA transcription in C. elegans. Genes & Development Jan 1;33(1-2):90-102. doi: 10.1101/gad.319293.118.
20. Chengming Zhu (#), Qi Yan (#), Chenchun Weng, Xinhao Hou, Hui Mao, Dun Liu, Xuezhu Feng (*) , Shouhong Guang (*) (2018) Erroneous ribosomal RNAs promote the generation of antisense ribosomal siRNA. PNAS 2018 Oct 2;115(40):10082-10087. doi: 10.1073/pnas.1800974115.
21. Fei Xu (#), Xuezhu Feng (#), Xiangyang Chen, Chenchun Weng, Qi Yan, Ting Xu, Minjie Hong, and Shouhong Guang (*)（2018）A cytoplasmic Argonaute protein promotes the inheritance of RNAi. Cell Reports. 2018 May 22;23(8):2482-2494.
22. Xufei Zhou (#), Xuezhu Feng (#), Hui Mao, Mu Li, Fei Xu, Kai Hu, and Shouhong Guang(*) (2017) RdRP-synthesized antisense ribosomal siRNAs silence pre-rRNA via the nuclear RNAi pathway. Nature Structural & Molecular Biology 2017 Mar;24(3):258-269. doi: 10.1038/nsmb.3376.
23. Hui Mao (#), Chengming Zhu (#), Dandan Zong, Chenchun Weng, Xiangwei Yang, Hui Huang, Dun Liu, Xuezhu Feng (*), and Shouhong Guang (*) (2015) The Nrde pathway mediates small RNA-directed histone H3 lysine 27 trimethylation in Caenorhabditis elegans. Current Biology 2015 Sep 21;25(18):2398-403.
24. Chen X (#), Li M (#), Feng X (*), Shouhong Guang (*) (2015) Targeted Chromosomal Translocations and Essential Gene Knockout Using CRISPR/Cas9 Technology in Caenorhabditis elegans. Genetics 2015 Dec;201(4):1295-306 doi: 10.1534/genetics.115.181883.
25. Zhou X (#), Xu F (#), Mao H, Ji J, Yin M, Feng X (*), and Shouhong Guang (*) (2014) Nuclear RNAi Contributes to the Silencing of Off-Target Genes and Repetitive Sequences in Caenorhabditis elegans. Genetics 2014 May;197(1):121-32.
26. Xiangyang Chen (#), Fei Xu (#), Chengming Zhu, Jiaojiao Ji, Xufei Zhou, Xuezhu Feng (*), and Shouhong Guang (*) (2014) Dual sgRNA-directed gene knockout using CRISPR/Cas9 technology in Caenorhabditis elegans. Scientific Reports 2014 Dec. 22;4:7581.
27.Guang, S., Bochner, A.F., Pavelec, D.M., Burkhart, K.B., Burton, N., and Kennedy, S., (2010) Small regulatory RNAs inhibit RNA Polymerase II during the elongation phase of transcription. Nature, 2010，Jun 24;465(7301):1097-101. doi: 10.1038/nature09095. Epub 2010 Jun 13.
28. Guang, S. (#), Bochner, A.F. (#), Pavelec, D.M., Burkhart, K.B., Harding, S., Lachowiec, J., and Kennedy, S., (2008) An Argonaute transports siRNAs from the cytoplasm to the nucleus. Science Jul 25;321(5888):537-41. doi: 10.1126/science.1157647. Erratum in: Science. 2009 Dec.
29.Guang, Shouhong, Felthauser, A., and Mertz, J. (2005) Binding of hnRNP L to the pre-mRNA processing enhancer (PPE) of herpes simplex virus’ thymidine kinase gene enhances both polyadenylation and nucleocytoplasmic export of intronless mRNAs. Mol. Cell. Biol. Aug;25(15):6303-13.
30. Guang, Shouhong and Mertz, J.E. (2005) PPE-like elements from intronless genes play additional roles in mRNA biogenesis than do ones from intron-containing genes. Nucleic Acid Res. Apr 20;33(7):2215-26. Print 2005.
联系方式：Tel: 0551-63607812 E-mail: sguang@@@ustc.edu.cn
实验室网页：http://staff.ustc.edu.cn/~sguang/index.html